| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Sinorhizobium medicae | TGAGCATCGTCGCCAACA | AGCGTTTCGATGATGCCG | 59.66 | 58.60 | 193 | 97 |
100.00%
|
100.00%
|
| Primer 12 | Sinorhizobium medicae | TGAGCATCGTCGCCAACA | TGGGTGAGCGTTTCGATGA | 59.66 | 59.33 | 199 | 94 |
100.00%
|
100.00%
|
| Primer 13 | Sinorhizobium medicae | TGAACGGTGTCACGCTGA | ACGGTCGCGATGTTCTGA | 59.19 | 59.04 | 247 | 94 |
100.00%
|
100.00%
|
| Primer 14 | Sinorhizobium medicae | TTCTGCTGGCTGTGGATGG | AGTTCGCGTCGCTTTTGC | 60.00 | 59.75 | 233 | 91 |
100.00%
|
100.00%
|
| Primer 15 | Sinorhizobium medicae | TGAACAGGCCATTCGGTCC | TTTCCACCCGTTGCTGGT | 60.00 | 59.40 | 248 | 90 |
100.00%
|
100.00%
|
| Primer 16 | Sinorhizobium medicae | TTTCGTCTCCTCGCTGCT | AGCCCACAAGAAGCAGCA | 58.63 | 59.48 | 251 | 90 |
100.00%
|
100.00%
|
| Primer 17 | Sinorhizobium medicae | CGCTTTTCGTCTCCTCGCT | AGCCCACAAGAAGCAGCA | 60.44 | 59.48 | 255 | 90 |
100.00%
|
100.00%
|
| Primer 18 | Sinorhizobium medicae | TTTTCGTCTCCTCGCTGCT | AGCCCACAAGAAGCAGCA | 59.33 | 59.48 | 252 | 90 |
100.00%
|
100.00%
|
| Primer 19 | Sinorhizobium medicae | ACGAATCGCTCCTCAAGGC | TCCGCAATGCTTCGACCA | 60.15 | 59.65 | 159 | 89 |
100.00%
|
100.00%
|
| Primer 20 | Sinorhizobium medicae | GCAGCAGACGTCGCTTTTC | TCCGCAATGCTTCGACCA | 60.15 | 59.65 | 178 | 89 |
100.00%
|
100.00%
|